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On the other hand, derepression regarding ThPOK transcription for the DP thymocytes away from ThPOKCD4.Sil rats mirrors expression away from endogenous Cd4 inside DP thymocytes regarding wt rats. Including, the newest SilThPOK prevents this new transcription of Cd4 when you look at the DP thymocytes out-of CD4ThPOK.Sil mice just like the inhibition out of endogenous ThPOK within the DP thymocytes away from wt rats. FACS studies off TCR?, CD69, CD4, and you can CD8 phrase from (b) total thymocytes, gated thymocytes and you will (c) peripheral T-phone subsets regarding wt rats or mutant lines, while the expressed. Mathematical importance is calculated per shown mutant line according to wt mice. FACS study from TCR?, CD69, CD4, and you will CD8 phrase out of complete thymocytes, gated thymocytes (c), and peripheral T-phone subsets (d) off wt mice otherwise mutant contours, as indicated.
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